Regulatory
p43-cre-B
Part:BBa_K5178001:Design
Designed by: Kaylin Shukert, Hannah Shen, Elizabeth MacPhail Group: iGEM24_Virginia (2024-10-01)
p43, cre binding sites version A
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Used a strong constitutive promoter to produce high levels of expression of downstream in the absence of glucose. Used two cre binding sites to enhance the inhibition effect of CcpA-HPrSerP complex on the downstream gene when glucose activates CcpA-HPrSerP.
Source
https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-13-401/tables/2