Regulatory
p43-cre-B

Part:BBa_K5178001:Design

Designed by: Kaylin Shukert, Hannah Shen, Elizabeth MacPhail   Group: iGEM24_Virginia   (2024-10-01)


p43, cre binding sites version A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used a strong constitutive promoter to produce high levels of expression of downstream in the absence of glucose. Used two cre binding sites to enhance the inhibition effect of CcpA-HPrSerP complex on the downstream gene when glucose activates CcpA-HPrSerP.


Source

https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-13-401/tables/2

References